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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
18/02/2019 |
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Data da última atualização: |
24/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
TRÍBULO, P.; BALZANO-NOGUEIRA, L.; CONESA, A.; SIQUEIRA, L. G. B.; HANSEN, P. J. |
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Afiliação: |
PAULA TRÍBULO, University of Florida, Gainesville, Florida; LEANDRO BALZANO-NOGUEIRA, University of Florida, Gainesville, Florida; ANA CONESA, University of Florida, Gainesville, Florida; LUIZ GUSTAVO BRUNO SIQUEIRA, CNPGL; PETER J. HANSEN, University of Florida, Gainesville, Florida. |
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Título: |
Changes in the uterine metabolome of the cow during the first 7 days after estrus. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Molecular Reproduction and Development, v. 86, n. 1, p. 75-87, 2019. |
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DOI: |
10.1002/mrd.23082 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract The uterine microenvironment during the first 7 days after ovulation accommodates and facilitates sperm transit to the oviduct and constitutes the sole source of nutrients required for the development of preimplantation embryos. Knowledge of the composition of uterine fluid is largely incomplete. Using untargeted mass spectrometry, we characterized the uterine metabolome during the first 7 days of the estrous cycle. Bovine uteri were collected on Days 0 (N = 4), 3 ( N = 4), 5 ( N = 3), and 7 ( N = 4) relative to ovulation and flushed with Dulbecco's phosphate-buffered saline. A total of 1,993 molecular features were detected of which 184 peaks with putative identification represent 147 unique metabolites, including amino acids, benzoic acids, lipid molecules, carbohydrates, purines, pyrimidines, vitamins, and other intermediate and secondary metabolites. Results revealed changes in the uterine metabolome as the cow transitions from ovulation to Day 7 of the estrous cycle. The majority of metabolites that changed with day reached maximum intensity on either Day 5 or 7 relative to ovulation. Moreover, several metabolites found in the uterine fluid have signaling capabilities and some have been shown to affect preimplantation embryonic development. In conclusion, the metabolome of the bovine uterus changes during early stages of the estrous cycle and is likely to participate in the regulation of preimplantation embryonic development. Data reported here will serve as the basis for future studies aiming to evaluate maternal regulation of preimplantation embryonic development and optimal conditions for the culture of embryos. MenosAbstract The uterine microenvironment during the first 7 days after ovulation accommodates and facilitates sperm transit to the oviduct and constitutes the sole source of nutrients required for the development of preimplantation embryos. Knowledge of the composition of uterine fluid is largely incomplete. Using untargeted mass spectrometry, we characterized the uterine metabolome during the first 7 days of the estrous cycle. Bovine uteri were collected on Days 0 (N = 4), 3 ( N = 4), 5 ( N = 3), and 7 ( N = 4) relative to ovulation and flushed with Dulbecco's phosphate-buffered saline. A total of 1,993 molecular features were detected of which 184 peaks with putative identification represent 147 unique metabolites, including amino acids, benzoic acids, lipid molecules, carbohydrates, purines, pyrimidines, vitamins, and other intermediate and secondary metabolites. Results revealed changes in the uterine metabolome as the cow transitions from ovulation to Day 7 of the estrous cycle. The majority of metabolites that changed with day reached maximum intensity on either Day 5 or 7 relative to ovulation. Moreover, several metabolites found in the uterine fluid have signaling capabilities and some have been shown to affect preimplantation embryonic development. In conclusion, the metabolome of the bovine uterus changes during early stages of the estrous cycle and is likely to participate in the regulation of preimplantation embryonic ... Mostrar Tudo |
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Palavras-Chave: |
Histotroph; Maternal-embryo crosstalk. |
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Thesaurus Nal: |
Endometrium; Metabolome. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02392naa a2200229 a 4500
001 2106149
005 2023-01-24
008 2019 bl uuuu u00u1 u #d
024 7 $a10.1002/mrd.23082$2DOI
100 1 $aTRÍBULO, P.
245 $aChanges in the uterine metabolome of the cow during the first 7 days after estrus.$h[electronic resource]
260 $c2019
520 $aAbstract The uterine microenvironment during the first 7 days after ovulation accommodates and facilitates sperm transit to the oviduct and constitutes the sole source of nutrients required for the development of preimplantation embryos. Knowledge of the composition of uterine fluid is largely incomplete. Using untargeted mass spectrometry, we characterized the uterine metabolome during the first 7 days of the estrous cycle. Bovine uteri were collected on Days 0 (N = 4), 3 ( N = 4), 5 ( N = 3), and 7 ( N = 4) relative to ovulation and flushed with Dulbecco's phosphate-buffered saline. A total of 1,993 molecular features were detected of which 184 peaks with putative identification represent 147 unique metabolites, including amino acids, benzoic acids, lipid molecules, carbohydrates, purines, pyrimidines, vitamins, and other intermediate and secondary metabolites. Results revealed changes in the uterine metabolome as the cow transitions from ovulation to Day 7 of the estrous cycle. The majority of metabolites that changed with day reached maximum intensity on either Day 5 or 7 relative to ovulation. Moreover, several metabolites found in the uterine fluid have signaling capabilities and some have been shown to affect preimplantation embryonic development. In conclusion, the metabolome of the bovine uterus changes during early stages of the estrous cycle and is likely to participate in the regulation of preimplantation embryonic development. Data reported here will serve as the basis for future studies aiming to evaluate maternal regulation of preimplantation embryonic development and optimal conditions for the culture of embryos.
650 $aEndometrium
650 $aMetabolome
653 $aHistotroph
653 $aMaternal-embryo crosstalk
700 1 $aBALZANO-NOGUEIRA, L.
700 1 $aCONESA, A.
700 1 $aSIQUEIRA, L. G. B.
700 1 $aHANSEN, P. J.
773 $tMolecular Reproduction and Development$gv. 86, n. 1, p. 75-87, 2019.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Florestas. Para informações adicionais entre em contato com cnpf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
23/09/2008 |
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Data da última atualização: |
23/09/2008 |
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Autoria: |
ROUGERIE, R.; DECAENS, T.; DEHARVENG, L.; CHIH-HAN, C.; JAMES, S.; PORCO, D.; HEBERT, P. |
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Título: |
DNA barcodes for soil animal taxonomy: transcending the final frontier. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
In: INTERNATIONAL COLLOQUIUM ON SOIL ZOOLOGY, 15; INTERNATIONAL COLLOQUIUM ON APTERYGOTA, 12., 2008, Curitiba. Biodiversity, conservation and sustainabele management of soil animal: abstracts. Colombo: Embrapa Florestas. Editors: George Gardner Brown; Klaus Dieter Sautter; Renato Marques; Amarildo Pasini. 1 CD-ROM. |
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Idioma: |
Inglês |
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Conteúdo: |
Soil animal communities comprise one of the largest biodiversity reservoirs in terrestrial
ecosystems and a very large proportion of species is undescribed. Soil biologists thus confront
a medium populated by probably millions of species whose discrimination remains an unfaced
challenge. While much soil biological research depends upon species diagnoses, taxonomic
expertise is collapsing. In fact, soil invertebrate identifications crystallize the four main
limitations pointed by Hebert et al. (2003): (1) that both phenotypic plasticity and genetic
variability in the characters employed for species recognition can lead to incorrect
identifications; (2) that this approach overlooks morphologically cryptic taxa, which are
common in many groups; (3) that since morphological keys are often effective only for a
particular life stage, gender or caste, many individuals cannot be identified; (4) that although
modern interactive versions represent a major advance, the use of keys often demands
such a high level of expertise that misdiagnoses are common. The aim of this review is to
present a promising approach to taxon recognition, which involves microgenomic identification
systems that involve discrimination of taxonomic units through the use of DNA sequences as
taxon ?barcodes?. The mitochondrial gene cytochrome c oxidase I (COI) has been identified
as a potential universal species barcode for animals. The taxonomic utility of this gene segment
has now been revealed in a broad range of taxonomic groups. It is used in an integrated
bioinformatics platform that supports all phases of the analytical pathway from specimen
collection to tightly validated barcode library (Ratnasingham & Hebert 2007). Current research
using this approach is in progress for soil animals and preliminary results on collembolans
and earthworms are used for illustration. We believe that barcoding of soil animals, as a
complementary tool for morphological approaches, would support soil biologists in species
identifications, stimulate accurate soil biodiversity surveys or any ecological research based
on species lists, and help soil systematists to solve taxonomic as well as phylogenetic
problems. MenosSoil animal communities comprise one of the largest biodiversity reservoirs in terrestrial
ecosystems and a very large proportion of species is undescribed. Soil biologists thus confront
a medium populated by probably millions of species whose discrimination remains an unfaced
challenge. While much soil biological research depends upon species diagnoses, taxonomic
expertise is collapsing. In fact, soil invertebrate identifications crystallize the four main
limitations pointed by Hebert et al. (2003): (1) that both phenotypic plasticity and genetic
variability in the characters employed for species recognition can lead to incorrect
identifications; (2) that this approach overlooks morphologically cryptic taxa, which are
common in many groups; (3) that since morphological keys are often effective only for a
particular life stage, gender or caste, many individuals cannot be identified; (4) that although
modern interactive versions represent a major advance, the use of keys often demands
such a high level of expertise that misdiagnoses are common. The aim of this review is to
present a promising approach to taxon recognition, which involves microgenomic identification
systems that involve discrimination of taxonomic units through the use of DNA sequences as
taxon ?barcodes?. The mitochondrial gene cytochrome c oxidase I (COI) has been identified
as a potential universal species barcode for animals. The taxonomic utility of this gene segment
has now been revealed in a broad range... Mostrar Tudo |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02973naa a2200193 a 4500
001 1314917
005 2008-09-23
008 2008 bl uuuu u00u1 u #d
100 1 $aROUGERIE, R.
245 $aDNA barcodes for soil animal taxonomy$btranscending the final frontier.
260 $c2008
520 $aSoil animal communities comprise one of the largest biodiversity reservoirs in terrestrial ecosystems and a very large proportion of species is undescribed. Soil biologists thus confront a medium populated by probably millions of species whose discrimination remains an unfaced challenge. While much soil biological research depends upon species diagnoses, taxonomic expertise is collapsing. In fact, soil invertebrate identifications crystallize the four main limitations pointed by Hebert et al. (2003): (1) that both phenotypic plasticity and genetic variability in the characters employed for species recognition can lead to incorrect identifications; (2) that this approach overlooks morphologically cryptic taxa, which are common in many groups; (3) that since morphological keys are often effective only for a particular life stage, gender or caste, many individuals cannot be identified; (4) that although modern interactive versions represent a major advance, the use of keys often demands such a high level of expertise that misdiagnoses are common. The aim of this review is to present a promising approach to taxon recognition, which involves microgenomic identification systems that involve discrimination of taxonomic units through the use of DNA sequences as taxon ?barcodes?. The mitochondrial gene cytochrome c oxidase I (COI) has been identified as a potential universal species barcode for animals. The taxonomic utility of this gene segment has now been revealed in a broad range of taxonomic groups. It is used in an integrated bioinformatics platform that supports all phases of the analytical pathway from specimen collection to tightly validated barcode library (Ratnasingham & Hebert 2007). Current research using this approach is in progress for soil animals and preliminary results on collembolans and earthworms are used for illustration. We believe that barcoding of soil animals, as a complementary tool for morphological approaches, would support soil biologists in species identifications, stimulate accurate soil biodiversity surveys or any ecological research based on species lists, and help soil systematists to solve taxonomic as well as phylogenetic problems.
700 1 $aDECAENS, T.
700 1 $aDEHARVENG, L.
700 1 $aCHIH-HAN, C.
700 1 $aJAMES, S.
700 1 $aPORCO, D.
700 1 $aHEBERT, P.
773 $tIn: INTERNATIONAL COLLOQUIUM ON SOIL ZOOLOGY, 15; INTERNATIONAL COLLOQUIUM ON APTERYGOTA, 12., 2008, Curitiba. Biodiversity, conservation and sustainabele management of soil animal: abstracts. Colombo: Embrapa Florestas. Editors: George Gardner Brown; Klaus Dieter Sautter; Renato Marques; Amarildo Pasini. 1 CD-ROM.
Download
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